Hemostatic materials are of great importance in medicine. However, their successful implementation is still challenging as it depends on two, often counteracting, attributes; achieving blood coagulation rapidly, before significant blood loss, and enabling subsequent facile wound-dressing removal, without clot tears and secondary bleeding. Here we illustrate an approach for achieving hemostasis, rationally targeting both attributes, via a superhydrophobic surface with immobilized carbon nanofibers (CNFs). We find that CNFs promote quick fibrin growth and cause rapid clotting, and due to their superhydrophobic nature they severely limit blood wetting to prevent blood loss and drastically reduce bacteria attachment. Furthermore, minimal contact between the clot and the superhydrophobic CNF surface yields an unforced clot detachment after clot shrinkage. All these important attributes are verified in vitro and in vivo with rat experiments. Our work thereby demonstrates that this strategy for designing hemostatic patch materials has great potential.To get more news about Bleeding Control Products, you can visit rusunmedical.com official website.

Uncontrolled hemorrhage and wound infection are leading causes of death in the medical field of wound care1,2,3. Improperly dressed wounds will prolong healing time and impose a high infection risk4, leading to significantly increased mortality and economic burden. To exemplify, $10 billion per year is spent on the treatment of complex wounds in North America5, while the global wound care market is estimated to reach $22 billion by 20206. Despite the progress in developing advanced hemostatic materials over the last few decades1,3,7,8,9, there are still two major challenges to be addressed: excessive blood loss during the period that the clot is forming and strong clot adhesion on the hemostatic dressing that causes pain, secondary bleeding, and possible infection during the wound-dressing removal.

The conventional method to deal with bleeding is mechanically pressing the wound with a cotton gauze10,11, which unavoidably absorbs blood and causes unnecessary blood loss and gauze adhesion onto the wound. Blood absorbed in the gauze forms a solid clot-gauze composite, forced peeling of which often tears the wound and causes secondary bleeding and pain. This makes it difficult to replace the old wound dressing without causing secondary infections or hemorrhage, in procedures ranging from common wounds to surgery, and to the extreme case of hemophilic patients12, where excessive bleeding will occur before coagulation. To deal with these problems, active clotting agents (chitosan3 or kaolin7) have been adopted into hemostatic materials, to reduce bleeding by expediting the coagulation process. However, such agents employ free micro-particles, which poses a safety threat of causing micro-thrombosis if they enter the vascular system13,14. Recently, researchers proposed using superhydrophobic (SHP) or superhydrophilic materials for hemostatic purposes. A superhydrophilic material (graphene sponge8) is reported to absorb water from the blood quickly, forming a dense layer of blood cells and platelets, thus promoting coagulation. Hydrophilic hemostatic material can also be prepared by spray coating β-chitosan on the porous nanofiber mat15, and the hydrophilic β-chitosan coating can increase blood wettability and thus enhance clotting. Alternatively, a SHP coating can be applied on the back of the normal superhydrophilic gauze as an impervious layer to prevent blood loss through the gauze9,16. However, the core functionality of these approaches is still either based on a blood-absorbing hemostatic material (superhydrophilic) that does not minimize blood loss and secondary bleeding or a blood-repelling material (superhemophobic) that simply repels blood but does not actively trigger clotting. Therefore, the aforementioned two key challenges on wound management still remain poorly addressed.

Here we report a strategy for achieving hemostasis by designing a SHP and blood-repelling surface that simultaneously achieves fast clotting with no blood loss, anti-bacterial property, and clot self-detachment. The non-wetting feature of the SHP hemostatic surface can withstand substantial blood pressure and help reduce blood loss and bacteria attachment. We find that carbon nanofibers (CNFs) immobilized on this surface can promote fast fibrin growth and thus clotting. Due to the presence of micro-air pockets within the blood-substrate contact area, there is minimal contact between the clot and the SHP CNF patch, leading to natural clot detachment after clot maturation and shrinkage, which reduces the peeling tension required to peel off the patch by about 1~2 orders of magnitude compared with a normal hydrophilic gauze or commercial hemostatic products. These features have been verified in vitro and in vivo, demonstrating the effectiveness of this strategy for designing hemostatic patch materials.